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  Indian J Med Microbiol
 

Figure 5: Effect of daylily flowers extracts on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophages. RAW264.7 macrophages (a and c) or J774A.1 macrophages (b and d) were incubated with daylily flowers aqueous extract, daylily flowers ethanolic extract or vehicle for 0.5 h, followed by stimulated with LPS for 24 h. The NO levels in the supernatants were analyzed by Griess reaction. (e) RAW264.7 macrophages were incubated with EF-1 to EF-6 or vehicle for 0.5 h, followed by stimulation with LPS for 24 h. The NO levels in the supernatants were analyzed by Griess reaction. *P < 0.05, **P < 0.01 and ***P < 0.001 compared to LPS-treated cells.

Figure 5: Effect of daylily flowers extracts on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophages. RAW264.7 macrophages (a and c) or J774A.1 macrophages (b and d) were incubated with daylily flowers aqueous extract, daylily flowers ethanolic extract or vehicle for 0.5 h, followed by stimulated with LPS for 24 h. The NO levels in the supernatants were analyzed by Griess reaction. (e) RAW264.7 macrophages were incubated with EF-1 to EF-6 or vehicle for 0.5 h, followed by stimulation with LPS for 24 h. The NO levels in the supernatants were analyzed by Griess reaction. *<i>P</i> < 0.05, **<i>P</i> < 0.01 and ***<i>P</i> < 0.001 compared to LPS-treated cells.