Close
  Indian J Med Microbiol
 

Figure 7: Repressive effect of sh-VPS9D1-AS1 on malignant phenotypes of prostate cancer cells was rescued through upregulating fibroblast growth factor-like-1 (FGFRL1). (a) Quantitative real-time reverse transcription polymerase chain reaction was utilized to examine FGFRL1 level in PC-3 cells of 4 groups (sh-NC + oe-NC, sh-VPS9D1-AS1 + oe-NC, sh-NC + oe-FGFRL1, and sh-VPS9D1-AS1 + oe-FGFRL1). (b) Western blot was employed for measuring FGFRL1 protein level in PC-3 cells of 4 groups. (c) MTT was done to test the proliferative ability of PC-3 cells in 4 groups. (d) Colony formation assay was conducted for evaluating the colony formation property of PC-3 cells in 4 groups. (e) A wound healing assay was introduced for examination of the migratory capacity of PC-3 cells in 4 groups (magnification: ×40). (f) Cell invasion assay was launched for testing the invasive capacity of PC-3 cells in 4 groups (magnification: ×100). *P < 0.05 relative to sh-NC + oe-NC group, #P < 0.05 relative to sh-NC + oe-FGFRL1 group, n = 3.

Figure 7: Repressive effect of sh-VPS9D1-AS1 on malignant phenotypes of prostate cancer cells was rescued through upregulating fibroblast growth factor-like-1 (FGFRL1). (a) Quantitative real-time reverse transcription polymerase chain reaction was utilized to examine FGFRL1 level in PC-3 cells of 4 groups (sh-NC + oe-NC, sh-VPS9D1-AS1 + oe-NC, sh-NC + oe-FGFRL1, and sh-VPS9D1-AS1 + oe-FGFRL1). (b) Western blot was employed for measuring FGFRL1 protein level in PC-3 cells of 4 groups. (c) MTT was done to test the proliferative ability of PC-3 cells in 4 groups. (d) Colony formation assay was conducted for evaluating the colony formation property of PC-3 cells in 4 groups. (e) A wound healing assay was introduced for examination of the migratory capacity of PC-3 cells in 4 groups (magnification: ×40). (f) Cell invasion assay was launched for testing the invasive capacity of PC-3 cells in 4 groups (magnification: ×100). *<i>P</i> < 0.05 relative to sh-NC + oe-NC group, <sup>#</sup><i>P</i> < 0.05 relative to sh-NC + oe-FGFRL1 group, <i>n</i> = 3.