Figure 2: Silencing VPS9D1-AS1 represses malignant phenotypes of prostate cells. (a) Impact of transfecting sh-VPS9D1-AS1 on VPS9D1-AS1 level in PC-3 cells was tested in quantitative real-time reverse transcription polymerase chain reaction assay. (b) Impact of silencing VPS9D1-AS1 on the proliferation of PC-3 cells was detected in MTT assay. (c) Impact of silencing VPS9D1-AS1 on the colony formation of PC-3 cells was assayed in a colony formation assay. (d) Impact of silencing VPS9D1-AS1 on the migration of PC-3 cells was evaluated in a wound healing assay (magnification: ×40). (e) Impact of silencing VPS9D1-AS1 on the invasion of PC-3 cells was estimated in cell invasion assay (magnification: ×100). *P < 0.05 relative to sh-NC group, n = 3.