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  Indian J Med Microbiol
 

Figure 3: Silencing NRAGE induces AML cell cycle arrest at G1 phase. (a-c) KG-1 and HL-60 cells were transfected with siNRAGE or siNC. (a) Flow cytometry showed the cell cycle phase distribution of KG-1 and HL-60 cells in the siNC and siNRAGE groups (n = 3). (b) Western blotting showed the protein levels of cyclin D1 of KG-1 and HL-60 cells in the siNC and siNRAGE groups (n = 3). (c) Western blotting showed the protein levels of CDK4 of KG-1 and HL-60 cells in the siNC and siNRAGE groups (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 versus the siNC group. NRAGE: Neurotrophin receptor-interacting melanoma-associated antigen homolog, AML: Acute myeloid leukemia, CDK4: Cyclin-dependent kinase 4.

Figure 3: Silencing NRAGE induces AML cell cycle arrest at G1 phase. (a-c) KG-1 and HL-60 cells were transfected with siNRAGE or siNC. (a) Flow cytometry showed the cell cycle phase distribution of KG-1 and HL-60 cells in the siNC and siNRAGE groups (<i>n</i> = 3). (b) Western blotting showed the protein levels of cyclin D1 of KG-1 and HL-60 cells in the siNC and siNRAGE groups (<i>n</i> = 3). (c) Western blotting showed the protein levels of CDK4 of KG-1 and HL-60 cells in the siNC and siNRAGE groups (<i>n</i> = 3). *<i>P</i> < 0.05, **<i>P</i> < 0.01 and ***<i>P</i> < 0.001 versus the siNC group. NRAGE: Neurotrophin receptor-interacting melanoma-associated antigen homolog, AML: Acute myeloid leukemia, CDK4: Cyclin-dependent kinase 4.