Figure 6: PA pretreatment activated SIRT1 and reversed OGD/R-induced alternations in the levels of apoptosis-related proteins in hepatocytes. (a) The expression levels of Bcl-2, Bax, cleaved-caspase 3/caspase 3 were detected by western blot. (b) Quantification of Bcl2 expression. (c) Quantification of Bax expression. (d) Quantification of cleaved-caspase 3/caspase 3 expression. The experiments were performed in triplicate. N = 3–5. ***P < 0.001 compared to Control; #P < 0.05, ##P < 0.01 and ###P < 0.001 compared to OGD/R; &P < 0.05 and &&&P < 0.001 compared to OGD/R + 80 μM PA. PA: Pachymic acid, SIRT1: Sirtuin 1, OGD/R: Oxygen-glucose deprivation/reperfusion.