Figure 5: LJJN does not affect Glu metabolism in Glu-induced FLSs. (a) FLSs were treated with increasing concentrations (from 5% to 20%) of LJJN (dissolved in DMSO) for 48 h. Cell viability was detected using CCK-8 assay. *P < 0.05 versus DMSO. (b) FLSs were stimulated by Glu to induce an injured synoviocytes model in vitro, with or without treatment with 15% LJJN for 48 h. Cell viability was detected using CCK-8 assay. (c) α-KG level was detected using its detection kit. (d) The mRNA level of GLUD2 was detected using qRT-PCR. (e) The protein expression of GLUD2 was detected by Western blot. **P < 0.01, ***P < 0.001 versus Control, #P < 0.05 versus Glu. LJJN: Lijie Capsules, FLSs: Fibroblast-like synoviocytes, qRT-PCR: Quantitative real-time polymerase chain reaction.