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  Indian J Med Microbiol
 

Figure 5: PCA reduces H2O2-induced proliferation and migration of RA-FLSs by activating Nrf2/Keap1 signaling pathway. (a) The viability of H2O2-induced MH7A cells treated by PCA and ML-385 was detected by CCK-8 assay. (b and c) The apoptosis of H2O2-induced MH7A cells treated by PCA and ML-385 was analyzed by TUNEL assay. (d and e) The migration of H2O2-induced MH7A cells treated by PCA and ML-385 was detected by wound healing assay. (f and g) The invasion of H2O2-induced MH7A cells treated by PCA and ML-385 was detected by transwell assay. (h) The expression of proteins associated with migration in H2O2-induced MH7A cells treated by PCA and ML-385 was detected by western blot. **P < 0.01 and ***P < 0.001 versus Control group. ###P < 0.001 versus H2O2 group. &P < 0.05, &&P < 0.01 and &&&P < 0.001 versus H2O2 + PCA group. PCA: protocatechuic acid, CCK-8: Cell counting kit-8, TUNEL: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.

Figure 5: PCA reduces H<sub>2</sub>O<sub>2</sub>-induced proliferation and migration of RA-FLSs by activating Nrf2/Keap1 signaling pathway. (a) The viability of H<sub>2</sub>O<sub>2</sub>-induced MH7A cells treated by PCA and ML-385 was detected by CCK-8 assay. (b and c) The apoptosis of H<sub>2</sub>O<sub>2</sub>-induced MH7A cells treated by PCA and ML-385 was analyzed by TUNEL assay. (d and e) The migration of H<sub>2</sub>O<sub>2</sub>-induced MH7A cells treated by PCA and ML-385 was detected by wound healing assay. (f and g) The invasion of H<sub>2</sub>O<sub>2</sub>-induced MH7A cells treated by PCA and ML-385 was detected by transwell assay. (h) The expression of proteins associated with migration in H<sub>2</sub>O<sub>2</sub>-induced MH7A cells treated by PCA and ML-385 was detected by western blot. **<i>P</i> < 0.01 and ***<i>P</i> < 0.001 versus Control group. <sup>###</sup><i>P</i> < 0.001 versus H<sub>2</sub>O<sub>2</sub> group. <sup>&</sup><i>P</i> < 0.05, <sup>&&</sup><i>P</i> < 0.01 and <sup>&&&</sup><i>P</i> < 0.001 versus H<sub>2</sub>O<sub>2</sub> + PCA group. PCA: protocatechuic acid, CCK-8: Cell counting kit-8, TUNEL: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.