Figure 5: GATA4 regulated SNHG1/miR-361-3p in Aβ1–42 fibril-infused rats. (a) Injection with sh-GATA4 virus reduced protein expression of Sp1 in Aβ-infused rats. (b) Injection with sh-GATA4 virus reduced SNHG1 expression and enhanced miR-361-3p in Aβ-infused rats. (c) Injection with Sp1 virus decreased the expression of miR-361-3p and increased Sp1 and SNHG1 in hippocampus of Aβ-infused rats. Loss of SNHG1 reduced the expression of SNHG1 and enhanced miR-361-3p. (d) Knockdown of SNHG1 decreased escape latency and increased number of platform crossings and time in target quadrant in Aβ-infused rats. (e) Overexpression of Sp1 attenuated GATA4 deficiency-induced decrease of escape latency, increase of number of platform crossings, and time in target quadrant in Aβ-infused rats. *** versus sham, P < 0.001. ##, ### versus Aβ + sh-NC or Aβ + sh-GATA4, P < 0.01, P < 0.001. GATA4: GATA-binding protein 4, SNHG1: Small nucleolar RNA host gene 1, sh-GATA4: shRNA targeting GATA4, Sp1: Specificity protein 1, sh-NC: negative control.