Figure 3: Raspberry ketone induced the browning of differentiated 3T3-L1 adipocytes. Western blotting analysis of the brown adipocyte markers (a) PR domain containing 16, (b) CD137, and (c) uncoupling protein 1 in raspberry ketone-treated differentiated 3T3-L1 adipocytes. Data are expressed as mean ± standard error of the mean (n = 3–4). *P < 0.05 compared with the control. (d) Immunofluorescence staining for uncoupling protein 1 and MitoTracker Deep Red FM in raspberry ketone-treated differentiated 3T3-L1 cells. The scale bars are 100 μm.