Figure 1: Representative photomicrographs of H and E-stained ovarian sections for control (a and b) and ischemia groups (c and d). (a) Primary multilaminar follicles (oval outline) depicting multiple layers of granulosa cells, secondary follicle (SF) showing follicular spaces (asterisks) and a primary oocyte (arrowhead). A mature Graafian follicle (GF) with evident basement membrane (1) and theca folliculi (2). Corpus luteum (CL) shows eosinophilic granulosa and theca lutein cells. Blood vessels (BV) are observed within the ovarian medulla. (b) A primordial follicle (arrow) is seen. A: antral space; GE: germinal epithelium. (c) Few growing follicles depicting vacuolated granulosa cells and separated theca folliculi (green arrow), atretic follicles (blue outline), cellular infiltration (arrowheads), CL exhibits vacuolated cells with dark shrunken nuclei. (d) Primordial follicles (blue outline) with degenerated oocytes. (a-d) Microscopic magnification x100.