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  Indian J Med Microbiol
 

Figure 5: Nicotinamide adenine dinucleotide promotes the expression of immediate-early response genes and increases neuronal Ca2+ levels in the frontal cortex of mice. (a) Injection of 8 mM nicotinamide adenine dinucleotide into the frontal cortex of mice obviously promoted the expression of C-Fos and Arc (n = 3). (b and c) Typical pictures of brain slices from the control and nicotinamide adenine dinucleotide groups after injection of AAV-GCaMP6. (d) Fluorescence intensity analysis of vector injection (n = 6). (e and f) Typical pictures of brain slices from control and nicotinamide adenine dinucleotide groups injected with GV581 containing the C-Fos promoter. (g) Fluorescence intensity analysis of GV581 injection (n = 6). The data in a were obtained by one-way ANOVA and bonferroni post-test. Data in d and g were obtained by unpaired t-test. *P < 0.05, **P < 0.01, ***P < 0.001. Scale = 200 μm.

Figure 5: Nicotinamide adenine dinucleotide promotes the expression of immediate-early response genes and increases neuronal Ca<sup>2+</sup> levels in the frontal cortex of mice. (a) Injection of 8 mM nicotinamide adenine dinucleotide into the frontal cortex of mice obviously promoted the expression of C-Fos and Arc (<i>n</i> = 3). (b and c) Typical pictures of brain slices from the control and nicotinamide adenine dinucleotide groups after injection of AAV-GCaMP6. (d) Fluorescence intensity analysis of vector injection (<i>n</i> = 6). (e and f) Typical pictures of brain slices from control and nicotinamide adenine dinucleotide groups injected with GV581 containing the C-Fos promoter. (g) Fluorescence intensity analysis of GV581 injection (<i>n</i> = 6). The data in a were obtained by one-way ANOVA and bonferroni post-test. Data in d and g were obtained by unpaired <i>t</i>-test. *<i>P <</i> 0.05, **<i>P <</i> 0.01, ***<i>P</i> < 0.001. Scale = 200 μm.