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  Indian J Med Microbiol
 

Figure 3: Wood vinegar from Griffith's ash inhibited NO generation in RAW264.7 macrophages. (a) RAW264.7 macrophages were incubated for 24 h with or without wood vinegar. Cell viability was measured by the alamarBlue assay. Data are expressed as mean ± SD of three independent experiments. *P < 0.05 compared to LPS alone (n = 3). (b) RAW264.7 macrophages were treated for 30 min with 0–4000-μg/mL wood vinegar, followed by incubation with or without 1-μg/mL LPS for 24 h. NO production in the culture medium was measured by the Griess reaction. The data are presented as the mean ± SD from three independent experiments. *P < 0.05 compared to LPS alone. LPS: Lipopolysaccharide, NO: Nitric oxide, SD: Standard deviation.

Figure 3: Wood vinegar from Griffith's ash inhibited NO generation in RAW264.7 macrophages. (a) RAW264.7 macrophages were incubated for 24 h with or without wood vinegar. Cell viability was measured by the alamarBlue assay. Data are expressed as mean ± SD of three independent experiments. *<i>P</i> < 0.05 compared to LPS alone (<i>n</i> = 3). (b) RAW264.7 macrophages were treated for 30 min with 0–4000-μg/mL wood vinegar, followed by incubation with or without 1-μg/mL LPS for 24 h. NO production in the culture medium was measured by the Griess reaction. The data are presented as the mean ± SD from three independent experiments. *<i>P</i> < 0.05 compared to LPS alone. LPS: Lipopolysaccharide, NO: Nitric oxide, SD: Standard deviation.