Figure 2: Silencing of KLHL23 reduces the formation of actin stress fibers and cell adhesion. (a) BFTC909-shEV and BFTC909-shKLHL23 cells were seeded into the wound healing chamber, and wound healing assay was performed. Actin and vinculin were detected by immunocytochemistry staining with phalloidin and anti-vinculin antibody. (b) Mean intensity of actin stress fibers was measured from cells (n = 12). (c) The intensity of merged actin and vinculin in intracellular cells was measured (n = 12). (d) The mean area of the merged vinculin in intracellular cells was measured (n = 12). The results were expressed as mean ± standard deviation. ***P < 0.005. Student's t-test with two-tailed P value. Scale bars: 10 μm.