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  Indian J Med Microbiol
 

Figure 2: Silencing of KLHL23 reduces the formation of actin stress fibers and cell adhesion. (a) BFTC909-shEV and BFTC909-shKLHL23 cells were seeded into the wound healing chamber, and wound healing assay was performed. Actin and vinculin were detected by immunocytochemistry staining with phalloidin and anti-vinculin antibody. (b) Mean intensity of actin stress fibers was measured from cells (n = 12). (c) The intensity of merged actin and vinculin in intracellular cells was measured (n = 12). (d) The mean area of the merged vinculin in intracellular cells was measured (n = 12). The results were expressed as mean ± standard deviation. ***P < 0.005. Student's t-test with two-tailed P value. Scale bars: 10 μm.

Figure 2: Silencing of KLHL23 reduces the formation of actin stress fibers and cell adhesion. (a) BFTC909-shEV and BFTC909-shKLHL23 cells were seeded into the wound healing chamber, and wound healing assay was performed. Actin and vinculin were detected by immunocytochemistry staining with phalloidin and anti-vinculin antibody. (b) Mean intensity of actin stress fibers was measured from cells (<i>n</i> = 12). (c) The intensity of merged actin and vinculin in intracellular cells was measured (<i>n</i> = 12). (d) The mean area of the merged vinculin in intracellular cells was measured (<i>n</i> = 12). The results were expressed as mean ± standard deviation. ***<i>P</i> < 0.005. Student's <i>t</i>-test with two-tailed<i> P </i>value. Scale bars: 10 μm.