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  Indian J Med Microbiol
 

Figure 1: Silencing of KLHL23 enhances the migration of urothelial cancer cells. (a) BFTC909 cells were infected with shEV or shKLHL23 virus for 48 h. After selection with puromycin, stable cells were seeded into the wound healing chamber for migration assay. (b) The BFTC909-shEV, BFTC909-shKLHL23 #1, and BFTC909-shKLHL23 #2 cells were performed migration for 18 h. The migrating cells were fixed and the distance of the wound was determined. (c) The wound distance in each sample was measured from eight fields and statistically analyzed using ImageJ software 1.51. (d) The silencing efficiency of shRNA was determined using quantitative real-time polymerase chain reaction. The results were expressed as mean ± standard deviation. ***P < 0.005. Student's t-test with two-tailed P value. Scale bars: 100 μm. (e and f) The migration and proliferation of cells were determined by using the real-time cell analysis assay for 18 h or 5 days of continuous tracking, respectively. The results were expressed as mean ± standard deviation and the slope was analyzed. *P < 0.05, ***P < 0.005. One-way ANOVA with Tukey multiple comparison.

Figure 1: Silencing of KLHL23 enhances the migration of urothelial cancer cells. (a) BFTC909 cells were infected with shEV or shKLHL23 virus for 48 h. After selection with puromycin, stable cells were seeded into the wound healing chamber for migration assay. (b) The BFTC909-shEV, BFTC909-shKLHL23 #1, and BFTC909-shKLHL23 #2 cells were performed migration for 18 h. The migrating cells were fixed and the distance of the wound was determined. (c) The wound distance in each sample was measured from eight fields and statistically analyzed using ImageJ software 1.51. (d) The silencing efficiency of shRNA was determined using quantitative real-time polymerase chain reaction. The results were expressed as mean ± standard deviation. ***<i>P</i> < 0.005. Student's <i>t</i>-test with two-tailed<i> P </i>value. Scale bars: 100 μm. (e and f) The migration and proliferation of cells were determined by using the real-time cell analysis assay for 18 h or 5 days of continuous tracking, respectively. The results were expressed as mean ± standard deviation and the slope was analyzed. *<i>P</i> < 0.05, ***<i>P</i> < 0.005. One-way ANOVA with Tukey multiple comparison.