Figure 2: Effects of linalyl acetate on NOX2 and p47phox subunit expression induced by exposure to OGD/R-only or calcium-related ischemic injury (CRII) conditions. (a) Relative expression of p47phox. (b) Relative expression of NOX2 protein, detected by western blotting. Glyceraldehyde 3-phosphate dehydrogenase protein was used as an internal control. Data are shown as means ± standard error of the mean (n = 3 per group). ##P < 0.01 and ###P < 0.001 versus the NOGD control group; *P < 0.05 and ***P < 0.001 versus the OGD/R-only group; +P < 0.05, ++P < 0.01 and +++P < 0.001 versus the CRII group. NOGD control group, no OGD/R in 1.5 mM [Ca2+]o; OGD/R-only group, oxygen-glucose deprivation/reoxygenation + dimethyl sulfoxide in 1.5 mM [Ca2+]o; OGD/R-LA500 group, OGD/R + 500 μM linalyl acetate in 1.5 mM [Ca2+]o; CRII group, OGD/R + dimethyl sulfoxide in 5 mM [Ca2+]o; CRII-LA500 group, OGD/R +500 μM linalyl acetate in 5 mM [Ca2+]o.