Chinese Journal of Physiology

ORIGINAL ARTICLE
Year
: 2023  |  Volume : 66  |  Issue : 1  |  Page : 28--35

Protocatechuic acid reduces H2O2-induced migration and oxidative stress of fibroblast-like synoviocytes in rheumatoid arthritis by activating Nrf2-Keap1 signaling pathway


Yan Liu1, Yucheng Zhang2, Keke Zhang3, Yue Wang1 
1 Department of Rheumatology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China
2 Department of Critical Care Medicine, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu, China
3 School of Integrated Traditional Chinese and Western Medicine, Jiangsu Health Vocational College, Nanjing, Jiangsu, China

Correspondence Address:
Dr. Yue Wang
Department of Rheumatology, Affiliated Hospital of Nanjing University of Chinese Medicine, No. 155, Hanzhong Road, Nanjing, 210029, Jiangsu
China

Honeycomb (Nidus vespae) is traditional Chinese medicine and can treat rheumatoid arthritis (RA), and protocatechuic acid (PCA) is a bioactive component of honeycomb. This study aimed to investigate whether PCA could reduce the H2O2-induced migration and oxidative stress of RA fibroblast-like synoviocytes (RA-FLSs). H2O2-induced RA-FLSs were used to simulate the in vitro model of RA. The viability, apoptosis, migration, invasion, and oxidative stress of RA-FLSs were detected by Cell Counting Kit-8 (CCK-8), terminal deoxynucleotidyl transferase dUTP nick-end labeling assay, wound healing, transwell assays, DCFDA staining, and malonaldehyde and superoxide dismutase enzyme-linked immunosorbent assay kits. The expression of migration and invasion-related proteins and Nrf2/Keap1 signaling pathway-related proteins was analyzed by western blotting. As a result, PCA suppressed the viability, migration, invasion, and oxidative and promoted apoptosis of H2O2-induced RA-FLSs by activating the Nrf2/Keap1 signaling pathway. ML-385, an Nrf2 inhibitor, could enhance the viability, migration, invasion, and oxidative and inhibited apoptosis of H2O2-induced RA-FLSs. In conclusion, PCA reduced H2O2-induced migration and oxidative stress of RA-FLSs by activating the Nrf2-Keap1 signaling pathway.


How to cite this article:
Liu Y, Zhang Y, Zhang K, Wang Y. Protocatechuic acid reduces H2O2-induced migration and oxidative stress of fibroblast-like synoviocytes in rheumatoid arthritis by activating Nrf2-Keap1 signaling pathway.Chin J Physiol 2023;66:28-35


How to cite this URL:
Liu Y, Zhang Y, Zhang K, Wang Y. Protocatechuic acid reduces H2O2-induced migration and oxidative stress of fibroblast-like synoviocytes in rheumatoid arthritis by activating Nrf2-Keap1 signaling pathway. Chin J Physiol [serial online] 2023 [cited 2023 Mar 24 ];66:28-35
Available from: https://www.cjphysiology.org/article.asp?issn=0304-4920;year=2023;volume=66;issue=1;spage=28;epage=35;aulast=Liu;type=0