Chinese Journal of Physiology

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 65  |  Issue : 2  |  Page : 64--71

Functional and structural assessment of the possible protective effect of platelet-rich plasma against ischemia/reperfusion-induced ovarian injury in adult rats


Eman Ahmed Allam1, Rehab Ahmed Abdel Moniem2, Gehan Yassin Soliman1 
1 Department of Medical Physiology, Faculty of Medicine, Alexandria University, Alexandria, Egypt
2 Department Histology and Cell Biology, Faculty of Medicine, Alexandria University, Alexandria, Egypt

Correspondence Address:
Dr. Eman Ahmed Allam
Department of Medical Physiology, Faculty of Medicine, Alexandria University, Alexandria
Egypt

This study aimed to evaluate the possible protective effect of platelet-rich plasma (PRP) on ischemia reperfusion (I/R)-induced ovarian injury in a rat model. Forty adult female albino rats were randomly assigned to four groups: control, ischemia, I/R, and I/R + intraperitoneal PRP. Induction of ischemia was done by bilateral ovarian torsion for 3 h, while reperfusion was done by subsequent detorsion for another 3 h. PRP was injected 30 min before detorsion. Histological assessment and measurement of ovarian anti-Mullerian hormone (AMH) were done to assess the degree of tissue damage and the remaining ovarian reserve. Ovarian malondialdehyde (MDA) and total antioxidant capacity (TAC) levels were measured to evaluate the oxidant-antioxidant balance. Tumor necrosis factor-α (TNF-α) was measured to assess degree of inflammation. Immunohistochemical assessment of ovarian vascular endothelial growth factor-A (VEGF-A) was also done. PRP treated I/R group revealed a significant decrease in MDA (P = 0.007), TNF-α (P = 0.001), and a significant increase in TAC (P = 0.001) and VEGF-A (P = 0.003) in comparison to the untreated I/R group. Furthermore, limited vascular congestion and inflammatory infiltration were observed after PRP treatment. However, no significant difference was detected in AMH after PRP treatment. Our results denoted that PRP may help in preservation of ovarian function and structure during surgical conservative detorsion of the torsioned ovary. These protective effects could be attributed to its ability to reduce oxidative stress, inflammation and also to its high content of growth factors especially VEGF.


How to cite this article:
Allam EA, Abdel Moniem RA, Soliman GY. Functional and structural assessment of the possible protective effect of platelet-rich plasma against ischemia/reperfusion-induced ovarian injury in adult rats.Chin J Physiol 2022;65:64-71


How to cite this URL:
Allam EA, Abdel Moniem RA, Soliman GY. Functional and structural assessment of the possible protective effect of platelet-rich plasma against ischemia/reperfusion-induced ovarian injury in adult rats. Chin J Physiol [serial online] 2022 [cited 2022 May 24 ];65:64-71
Available from: https://www.cjphysiology.org/article.asp?issn=0304-4920;year=2022;volume=65;issue=2;spage=64;epage=71;aulast=Allam;type=0