| ORIGINAL ARTICLE |
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| Year : 2023 | Volume
: 66
| Issue : 2 | Page : 103-110 |
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MiR-598-5p inhibits breast cancer tumor growth and lung metastasis by targeting PPAPDC1A
Xinyi Guo1, Fan Yang2, Liangfei Yu2, Ronglan Wen2, Xin Zhang2, Hui Lin2
1 Department of Breast Surgery, Affiliated Fuzhou First Hospital of Fujian Medical University; Department of General Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, China
2 Department of Breast Surgery, Affiliated Fuzhou First Hospital of Fujian Medical University, Fuzhou, Fujian Province, China
Correspondence Address:
Dr. Hui Lin
Department of Breast Surgery, Affiliated Fuzhou First Hospital of Fujian Medical University, 190 Dadao Road, Taijiang District, Fuzhou 350009, Fujian Province
China
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/cjop.CJOP-D-22-00089
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This study aimed to explore the effects of PPAPDC1A on the malignant phenotype of breast cancer (BC) in vivo and in vitro. PPAPDC1A expression was examined in BC tissues and cell lines by real-time polymerase chain reaction and Western blot. In this article, cell proliferation was evaluated by Cell Counting Kit-8 assay and colony formation assay, and cell migration and invasion were evaluated by wound healing assay and transwell assays. Furthermore, in vivo cell growth and pulmonary metastasis experiments were also performed using nude mice. The results showed that compared with normal tissues and cells, the PPAPDC1A expression in BC tissues and cell lines were both significantly increased. The PPAPDC1A targeting sequence significantly inhibited the PPAPDC1A expression and cell proliferation, migration, and invasion. The results of xenograft showed that knockdown of PPAPDC1A inhibited tumor growth and lung metastasis of BC. Then, the Dual-Luciferase Reporter Assay confirmed that miR-598-5p targeted the regulation of PPAPDC1A expression. In addition, the miR-598-5p expression in BC tissues was lower than that in the normal tissues. The rescue experiment showed that PPAPDC1A overexpression reversed the inhibitory effect of miR-598-5p mimic on cell proliferation, migration, and invasion. In conclusion, PPAPDC1A was highly expressed in BC tissues and cell lines, and miR-598-5p inhibited the malignant phenotype of BC by targeting PPAPDC1A.
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