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Antrodia salmonea extract inhibits cell proliferation through regulating cell cycle arrest and apoptosis in prostate cancer cell lines
Pang-Ting Cheng1, Yu-Chiao Cheng1, Muhammet Oner1, Yu-Hsuan Li2, Mei-Chih Chen3, Jyh-Horng Wu4, Ting-Chieh Chang1, Ayse Celik1, Fang-Ling Liu1, Hsin-Yi Wang5, Chih-Ho Lai6, Jer-Tsong Hsieh7, Chieh-Yin Chen8, Ho Lin9
1 Department of Life Sciences, National Chung Hsing University, Taichung City, Taiwan
2 Department of Life Sciences, National Chung Hsing University, Taichung City; Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, Taichung City, Taiwan
3 Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, Taichung City, Taiwan
4 Department of Forestry, National Chung Hsing University, Taichung City, Taiwan
5 Department of Nuclear Medicine, Taichung Veterans General Hospital, Taichung City, Taiwan
6 Department of Microbiology and Immunology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan City, Taiwan
7 Department of Urology, University of Texas Southwestern Medical Center, Dallas, TX, USA
8 Experimental Forest, College of Bio-Resources and Agriculture, National Taiwan University, Taichung City, Taiwan
9 Department of Life Sciences; Ph.D. Program in Translational Medicine; Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung City, Taiwan
Correspondence Address:
Dr. Chieh-Yin Chen
Experimental Forest, College of Bio-Resources and Agriculture, National Taiwan University, Taichung City
Taiwan
Prof. Ho Lin
Department of Life Sciences, National Chung Hsing University, Taichung City
Taiwan
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/cjp.cjp_78_21
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Antrodia salmonea (AS) is a fungus, which belongs to a fungal family of Taiwanofungus salmoneus with the features of anti-oxidant, anti-inflammatory, and anticancer. Recent studies have shown that AS has anti-cancer functions in ovarian and breast cancer. However, the effects of AS on prostate cancer (PCa) proliferation remain unknown. Therefore, we investigated the role of AS in PCa proliferation through apoptosis, and cell cycle regulation in PCa cell lines. Our results showed that Antrodia salmonea extract (ASE) inhibited PCa cells growth with a dose-dependent manner. In addition, ASE decreased the anchorage-independent growth formation ability in PC3 cells. Moreover, ASE-induced cell growth inhibition in PCa cells (DU145, PC3) was correlated to decreased cell cycle-related proteins such as cyclin A/B and cyclin-dependent kinase CDK1/2/4, and increased cell cycle inhibitor proteins p21. Besides, ASE decreased the total protein level of epidermal growth factor receptor and its downstream signaling pathways Akt and Erk in both PCa cells. We found that apoptotic markers such as cleaved-PARP protein levels increased significantly in DU145 cells indicating ASE might induce apoptosis. In conclusion, our results suggest that ASE may have the ability to induce PCa cell death through regulating cell cycle arrest and apoptosis pathways.
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